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Journal: bioRxiv

Article Title: Defective BRCA1-mediated DNA end resection drives tandem duplication formation and FANCM synthetic lethality

doi: 10.64898/2026.02.20.706968

Figure Lengend Snippet:

Article Snippet: Cas9-sgRNA target sites in the mouse genome sequence were identified using the Heidelberg CCtop tool: https://cctop.cos.uni-heidelberg.de . sgRNA in vitro synthesis was performed using the Engen sgRNA Synthesis Kit (New England Biolabs, E3322S) and purified using the Clean and Concentrate Kit (Zymo Research, R1017). sgRNA was electrophoresed on a denaturing gel (Novex 10% TBE-Urea; ThermoFisher Scientific, EC6875BOX), to assess quality prior to transfection.

Techniques: Sequencing